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Antibodies sure to the blocking peptide now not bind into the epitope on the focus on protein. By comparing the staining within the blocked antibody as opposed to the antibody on your own, one can see which staining is precise.
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This guide outlines further more attainable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
This guideline outlines more doable optimization of cellular and immunoassay parameters to make certain the very best results are...
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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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Blocking peptides are peptides that bind specially to the focus on antibody and block antibody binding. These peptide typically consists of the epitope acknowledged by the antibody.
Target Information and facts Cyclin E belongs into the highly get more info conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation designs which contribute on the temporal coordination of each and every mitotic party. Cyclin E varieties a complex with and capabilities for a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress through S period.
Overexpression of this gene continues to be noticed in lots of tumors, which leads to chromosome instability, and therefore may perhaps lead to tumorigenesis. This protein was discovered to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a crucial part in promoting cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have already been explained. Two supplemental splice variants were noted but comprehensive nucleotide sequence details is just not but offered.
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